Molecular Motors and a Spectrin Matrix Associate with Golgi Membranes In Vitro
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چکیده
منابع مشابه
Molecular Motors and a Spectrin Matrix Associate with Golgi Membranes In Vitro
Cytoplasmic dynein is a microtubule minus-end-directed motor that is thought to power the transport of vesicles from the TGN to the apical cortex in polarized epithelial cells. Trans-Golgi enriched membranes, which were isolated from primary polarized intestinal epithelial cells, contain both the actin-based motor myosin-I and dynein, whereas isolated Golgi stacks lack dynein but contain myosin...
متن کاملMicrotubule plus ends, motors, and traffic of Golgi membranes.
The intimate link between microtubule (MT) organization and the components of the secretory pathway has suggested that MT-based motility is an essential component of vesicular membrane transport and membrane polarization. The molecular details of these processes are still under investigation; however, a novel class of MT plus end-binding proteins shed new light on transport between the endoplas...
متن کاملMolecular motors are differentially distributed on Golgi membranes from polarized epithelial cells
Microtubules (MT) are required for the efficient transport of membranes from the trans-Golgi and for transcytosis of vesicles from the basolateral membrane to the apical cytoplasm in polarized epithelia. MTs in these cells are primarily oriented with their plus ends basally near the Golgi and their minus-ends in the apical cytoplasm. Here we report that isolated Golgi and Golgi-enriched membran...
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Spectrin is a major component of a membrane-associated cytoskeleton involved in the maintenance of membrane structural integrity and the generation of functionally distinct membrane protein domains. Here, we show that a homolog of erythrocyte beta-spectrin (beta I sigma*) co-localizes with markers of the Golgi complex in a variety of cell types, and that microinjected beta-spectrin codistribute...
متن کاملGolgi Spectrin: Identification of an Erythroid/3-Spectrin Homolog Associated with the Golgi Complex
Spectrin is a major component of a membrane-associated cytoskeleton involved in the maintenance of membrane structural integrity and the generation of functionally distinct membrane protein domains. Here, we show that a homolog of erythrocyte/3-spectrin (/3IE*) co-localizes with markers of the Golgi complex in a variety of cell types, and that microinjected/~-spectrin codistributes with element...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1997
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.139.5.1169